transcriptome shotgun assembly (tsa) sequences  (Pacific Biosciences)

Journal: Cell Proliferation

Article Title: Promyelocytic leukaemia zinc finger maintains self‐renewal of male germline stem cells ( mGSC s) and its expression pattern in dairy goat testis

doi: 10.1111/cpr.12048

Figure Lengend Snippet: RT ‐ PCR analysis of expression of PLZF and associated markers in dairy goat testicular tissues of 30 dpp, 6 M and 10 M samples. (a) Expression of PLZF, OCT4, STRA8, CDk2, CYCIND1 and C‐MYC in 30 dpp, 6 M and the 10 M testicular tissues, β‐actin as internal control. (b) Relative value of (a) (measured by ImageJ). **P < 0.01. (c) Series of goat homology TSA sequences gained by the Bos taurus seed sequence BLAST alignment in the goat TSA database.

Article Snippet: Fluorescence signal was collected every 0.5 °C for 10 s. In silico cloning and evolutionary relationship of PLZF coding domain sequence (CDS) Transcriptome Shotgun Assembly (TSA) sequences Database in NCBI (National Center for Biotechnology Information) were used for in silico cloning, splicing and obtaining dairy goat PLZF, which contains the whole CDS.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Sequencing

Journal: Cell Proliferation

Article Title: Promyelocytic leukaemia zinc finger maintains self‐renewal of male germline stem cells ( mGSC s) and its expression pattern in dairy goat testis

doi: 10.1111/cpr.12048

Figure Lengend Snippet: Identification of dairy goat PLZF gene. (a) Agarose gel electrophoresis analysis of PLZF PCR product. (b) pPLZF‐IRES2‐EGFP was digested by SalI and BamHI. M1: 15 000 bp ladder; M3: 10 000 bp ladder; M2: 2000 bp ladder. pIRES2‐EGFP eukaryotic expression vector was in the order of 5300 bp and PLZF fragment was around 2300 bp. (c) Construction of phylogenetic trees with PLZF cDNA sequence of dairy goat and other species. (d) The homologic comparison of Plzf cDNA sequence of dairy goat compare with sequences published in NCBI GenBank.

Article Snippet: Fluorescence signal was collected every 0.5 °C for 10 s. In silico cloning and evolutionary relationship of PLZF coding domain sequence (CDS) Transcriptome Shotgun Assembly (TSA) sequences Database in NCBI (National Center for Biotechnology Information) were used for in silico cloning, splicing and obtaining dairy goat PLZF, which contains the whole CDS.

Techniques: Agarose Gel Electrophoresis, Expressing, Plasmid Preparation, Sequencing, Comparison